Table 2.
Antibacterial activity of goat cathelicidins and melittin.
| Strain | sbmA/yaiW∗∗ | Minimum inhibitory concentration (μM)∗ |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Melittin |
ChMAP-28 |
mini-ChBac7.5Nα |
mini-ChBac7.5Nα(1–16) |
||||||
| Without NaCl | 0.9% NaCl | Without NaCl | 0.9% NaCl | Without NaCl | 0.9% NaCl | Without NaCl | 0.9% NaCl | ||
| M. luteus B-1314 | -/- | 0.25 | 0.5 | 0.25 | 0.5 | 0.125 | 0.5 | 1 | 8 |
| B. subtilis B-886 | -/- | 0.5 | 0.5 | 0.25 | 1 | 0.25 | 4 | 1 | >32 |
| E. faecalis ATCC 29212 | -/- | 1 | 1 | 4 | >8 | >32 | >32 | >32 | >32 |
| S. aureus ATCC 29213 | -/- | 1 | 1 | 1 | 2 | 8 | >32 | >32 | >32 |
| S. aureus 209P | -/- | 2 | 16 | 0.06 | 0.5 | 2 | 16 | 8 | >32 |
| E. coli C600 | +/+ | 4 | 8 | 0.06 | 0.125 | 2 | 4 | 2 | 16 |
| E. coli ML-35p | +/+ | 2 | 8 | 0.06 | 0.06 | 0.5 | 4 | 0.5 | 8 |
| P. aeruginosa PAO1 | -/- | 4 | 8 | 0.25 | 1 | 2 | >32 | 16 | >32 |
| A. baumannii (XDR CI 2675) | +/- | 2 | 8 | 0.03 | 0.25 | 2 | >32 | 4 | >32 |
| K. pneumoniae (CI 287) | +/+ | 4 | 16 | 0.125 | 0.5 | 4 | 16 | 4 | >32 |
| E. cloacae (XDR CI 4172) | +/+ | 2 | 8 | 0.125 | 0.25 | 1 | 4 | 1 | >32 |
∗Antibacterial testing was performed in the Mueller-Hinton broth at 37°C.∗∗The presence of genes encoding the membrane transporters SbmA and YaiW that affect sensitivity to proline-rich antimicrobial peptides (Arnold et al., 2014).