Table 3.
Synergy between goat cathelicidins ChMAP-28 and mini-ChBac7.5Nα.
| Strain | ChMAP-28 |
mini-ChBac7.5Nα |
FICI∗ | Synergy | ||||
|---|---|---|---|---|---|---|---|---|
| MICA | [A] | FICA | MICB | [B] | FICB | |||
| E. coli BL21 (DE3) | 0.06 | 0.015 | 0.25 | 8 | 1 | 0.125 | 0.375 | Yes |
| E. coli ML-35p | 0.06 | 0.008 | 0.125 | 4 | 1 | 0.25 | 0.375 | Yes |
| E. coli C600 | 0.125 | 0.015-0.03 | 0.125-0.25 | 4 | 1 | 0.25 | 0.375-0.5 | Yes |
| E. coli (XDR CI 1057) | 0.125 | 0.008 | 0.063 | 8 | 2 | 0.25 | 0.313 | Yes |
| E. coli (CI 214) | 0.06 | 0.015 | 0.25 | >64 | 4 | 0.031 | 0.281 | Yes |
| E. cloacae (XDR CI 4172) | 0.25 | 0.03 | 0.125 | 4 | 0.5 | 0.125 | 0.25 | Yes |
| K. pneumoniae (CI 287) | 0.5 | 0.06 | 0.125 | 16 | 2 | 0.125 | 0.25 | Yes |
| A. baumannii (XDR CI 2675) | 0.25 | 0.03 | 0.125 | >32 | 0.5 | 0.008 | 0.133 | Yes |
| P. aeruginosa PAO1 | 0.125 | 0.06 | 0.5 | >32 | 8 | 0.125 | 0.625 | No |
| S. aureus 209P | 0.5 | 0.03 | 0.063 | 16 | 2 | 0.125 | 0.188 | Yes |
∗The estimation of synergistic effects between cathelicidins was performed by calculating the fractional inhibitory concentration index (FICI) according to the equation: FICI = FICA + FICB = [À]/MICÀ + [B]/MICB, where MICÀ and MICB are the MICs of individual peptides, while [A] and [B] are the MICs of A and B when used together. A synergistic effect was defined at a FICI ≤ 0.5.