Figure 2.

(A) Human monocytes were incubated for 24 h in RPMI, β-glucan or LPS. Whereas, the former was the control, the latter two treatments represent the trained and tolerant cells, respectively. Subsequently, the cells were left for 5 days in RPMI medium with 10% human pooled serum and BrdU. Upon completion of this period, the amount of BrdU (as a parameter of endoreplication) was quantified by a colorimetric assay, and raw OD data were recorded. N = 6, Wilcoxon; ^*p < 0.05; ^***p < 0.001. (B,C) Human monocytes were incubated for 24 h in RPMI, β-glucan or LPS (the former being the control, the latter two the trained and tolerant cells, respectively). Afterwards, the cells were left for 4 days and then RNA sequencing was performed on these cells without further treatment. Data are expressed as reads per kilobase per million reads (RPKM). N = 3, Wilcoxon; ^*p < 0.05; ^**p < 0.01.