Table 1.
Primer sequence and length of the amplified product.
| Gene | Forward primer | Reverse primer | Cells | Product size |
|---|---|---|---|---|
| CTL4 | CAATCGCAAAATACAGCTCGTG | CCAGTAGGACGAGGAACGAAG | Mosquito | 231 Bp |
| CTL6 | CTGGATGCGTACTTTGAATGC | CAAAGGTCCTCTTTGCGATCA | Mosquito | 116 Bp |
| HNT | CGTAGTGCCTGTCCCAAACT | ATTGTTGCCGCTGCTCT | Mosquito | 125 Bp |
| TEP1 | GTGAACTTGCCGAGTGGCTA | CGACAGTAGTACCACCGTAGAGG | Mosquito | 106 Bp; 185 Bp* |
| PPO1 | GGCGGACCAAATCAAGCAG | CGATTGCCCGATTCGTCAAC | Mosquito | 102 Bp; 185 Bp* |
| LRIM1 | CGTGCTCGCTAGCTACGTT | CGTAGTGCCTGT CCCAAACT | Mosquito | 117 Bp |
| HK2 | GAGCTCAATTCTGTGTGGAGT | ACTTCTTGAGAACTATGTACCCTT | Monocytes | 77 Bp |
| PFKP | CGAAGGCGATGGGGTGAC | CATCGCTTCGCCACCTTTC | Monocytes | 75 Bp |
| TNFA | GTGCTTGTTCCTCAGCCTCT | ATCACTCCAAAGTGCAGCAG | Monocytes | 81 Bp |
| IL6 | AGGGAGAGCCAGAACACAGA | GAGTTTCCTCTGACTCCATCG | Monocytes | 97 Bp |
CTL4, C-type lectin 4; CTL6, C-type lectin 6; HNT, Hindsight, TEP-1, thioester-containing protein 1; PPO1, prophenoloxidase 1; LRIM1, leucine-rich immune protein; HK2, hexokinase 2; PFKP, phosphofructokinase; TNFA, tumor necrosis factor; IL6, interleukin 6.
*
Amplified product length in the genomic sequence. The primers were designed between two exons to reveal the genomic product and the product of the transcript in DNA synthesis as well as the gene expression level.