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. 2018 Dec 5;38(49):10566–10581. doi: 10.1523/JNEUROSCI.1196-18.2018

Figure 5.

Figure 5.

Effective retrograde transduction of corticospinal tract neurons after spinal injury. A, Adult female mice received unilateral cervical dorsal hemisection or sham injury, followed by bilateral injection of mixed Retro-AAV-tdTomato and CTB-488. B–E, In sagittal sections of spinal cord 28 d postinjection, GFAP immunohistochemistry (B) and the absence of CTB-488 (C) and tdTomato (D) signal caudal to the injury confirmed complete dorsal transections. F, G, Coexpression of CTB-488 and tdTomato was high in CST neurons in injured animals (F) and in both cortices of animals that received unilateral spinal injury (G). H, Quantification of coexpression rates showed no difference in the left cortex of injured animals, the location of axotomized CST cell bodies (p = 0.82, two-way ANOVA with post hoc Tukey's). N = 4 animals per group, >200 individual cells quantified per animal. Error bars show SEM. Scale bars, 1 mm.