Table 1.

Kinetic parameters of E. histolytica dephospho CoA kinase 1 (EhDPCK1) and dephospho CoA kinase 2 (EhDPCK2).

Enzyme	Substrate	Km (μM)	Vmax (μmole/min/mg)	Kcat (min-1)	Kcat/Km (min-1μM-1)
EhDPCK1	Dephospho CoA	114 ± 19	3.71 ± 0.43	88.9 ± 10.3	0.78 ± 0.04
	ATP	19.6 ± 1.2	3.54 ± 0.09	84.8 ± 2.4	4.32 ± 0.23
EhDPCK2	Dephospho CoA	57.9 ± 6.07	2.48 ± 0.15	57.5 ± 3.5	0.99 ± 0.07
	ATP	15.0 ± 2.4	2.71 ± 0.17	62.9 ± 3.7	4.25 ± 0.74

Assays were performed in the presence 10 mM MgCl2, 15 mM HEPES, 20 mM NaCl, 1 mM EGTA, 0.02% Tween-20, 0.1 mg/mL β-globulins, and 4–256 μM dephospho-CoA to determine kinetic parameters toward ATP or 5–100 μM ATP to determine kinetic parameters toward dephospho-CoA. Reactions were conducted at 37°C at pH 8 for 60 min. Mean ± SE of three replicates are shown.