Table 2.

Phosphoryl donor specificity^a of EhDPCK1 and EhDPCK2.

Phosphoryl donorb	Relative activity (%)
	EhDPCK1	EhDPCK2
ATP	100.0 ± 0.0	100.0 ± 0.0
TTP	24.1 ± 2.2	4.9 ± 1.1
GTP	32.6 ± 2.6	33.0 ± 3.8
CTP	15.4 ± 1.8	7.3 ± 1.6
UTP	2.6 ± 0.1	4.5 ± 0.3
None	ND	ND

^aAssays were performed in the presence 10 mM MgCl₂, 15 mM HEPES, 20 mM NaCl, 1 mM EGTA, 0.02% Tween-20, 0.1 mg/mL β-globulins and 1 mM dephospho-CoA. Reactions were conducted at 37°C in pH 8 for 60 min. ^bThe final concentration used was 0.1 mM. The activity is shown in percentage (%) relative to that toward ATP. ND, not detected. Mean ± SE of three replicates are shown.