Table 12.

Effect of dietary inclusion of an organic acids-based formulation (OABF) and avilamycin dietary inclusion on relative mRNA levels of ileal mucosa barrier genes of 42-day-old broilers.

Item		MUC2	IgA	ZO1	ZO2	CLDN1	CLDN5	OCLN	NFKB1	TLR2B	TLR4
Main effect
OABF1	No	1.196	2.925	1.89	1.52	1.32	1.79	1.52	1.23	1.97	2.07
	Yes	0.851	3.426	1.00	1.10	1.38	0.83	0.99	1.24	1.47	1.16
Avilamycin2	No	1.019	3.412	1.88	1.42	1.64	1.86	1.60	1.55	2.49	2.25
	Yes	1.028	2.938	1.01	1.20	1.05	0.77	0.91	0.92	0.96	0.97
Treatments3
CON		1.049	3.753	2.82a	2.03a	2.06a	2.67a	2.14	1.74	3.06	3.11
OA		0.988	3.072	0.94b	0.81b	1.23ab	1.04b	1.07	1.35	1.92	1.39
OAAV		0.714	3.780	1.07b	1.40ab	1.53ab	0.62b	0.91	1.12	1.02	0.92
AV		1.343	2.096	0.95b	1.02b	0.57b	0.92b	0.90	0.72	0.89	1.02
Statistics
SEM4		0.148	0.770	0.344	0.341	0.334	0.331	0.295	0.262	0.565	0.490
PO		0.027	0.520	0.016	0.228	0.859	0.003	0.169	0.974	0.381	0.073
PA		0.947	0.543	0.016	0.533	0.085	0.001	0.018	0.024	0.011	0.014
PO×A		0.065	0.136	0.007	0.027	0.012	0.036	0.125	0.140	0.272	0.110

MUC2 = mucin 2; IgA = immunoglobulin A; ZO1 = zonula occludens-1; ZO2 = zonula occludens-2; CLDN1 = claudin-1; CLDN5 = claudin-5; CLDN = occludin; NFKB1 = nuclear factor kappa B subunit 1; TLR2B = toll-like receptor 2 family member B; TLR4 = toll-like receptor 4.

^{a, b} Interaction means within the same column differ significantly (P ≤ 0.05).

¹No: no OABF addition; Yes: addition of 1 g OABF/kg diet.

²No: no avilamycin addition; Yes: addition of 2.5 mg of avilamycin/kg diet.

³Interaction means from 8 replicate pens per treatment. Treatments include: control – no additions (CON), 1 g OABF/kg diet (OA), avilamycin 2.5 mg active components/kg diet (AV) and combination of OA + AV (OAAV).

⁴Pooled standard error of means.