Table 3.
Oligonucleotide primers used for quantitative RT-PCR.
| Target | Primer sequence (5′ to 3′) | Annealing temperature, °C | PCR product size, bp | GenBank accession No. |
|---|---|---|---|---|
| GAPDH | F: GCTGAATGGGAAGCTTACTG R: AAGGTGGAGGAATGGCTG |
60 | 216 | NM_204305.1 |
| MUC2 | F:TCACCCTGCATGGATACTTGCTCA R:TGTCCATCTGCCTGAATCACAGGT |
62 | 228 | NM_001318434.1 |
| IgA | F:GTCACCGTCACCTGGACTACA R:ACCGATGGTCTCCTTCACATC |
60 | 192 | S40610 |
| ZO1 | F:TAAAGCCATTCCTGTAAGCC R:GTTTCACCTTTCTCTTTGTCC |
60 | 243 | XM_015278975.1 |
| ZO2 | F:GTTTCACCTTTCTCTTTGTCC R:TAAAGCCATTCCTGTAAGCC |
60 | 239 | NM_204918.1 |
| CLDN1 | F:CTGATTGCTTCCAACCAG R:CAGGTCAAACAGAGGTACAAG |
59 | 140 | NM_001013611.2 |
| CLDN5 | F:CATCACTTCTCCTTCGTCAGC R:GCACAAAGATCTCCCAGGTC |
59 | 111 | NM_204201.1 |
| OCLN | F:TCATCGCCTCCATCGTCTAC R:TCTTACTGCGCGTCTTCTGG |
62 | 240 | NM_205128.1 |
| NFKB1 | F: TGTGGTTGTCAGGATGGTC R: GGTCTGGTAAAGGTCATTTCTC |
62 | 273 | NM_205134 |
| TLR2B | F:CTTGGAGATCAGAGTTTGGA R:ATTTGGGAATTTGAGTGCTG |
62 | 238 | NM_001161650.1 |
| TLR4 | F: GTCTCTCCTTCCTTACCTGCTGTTC R:AGGAGGAGAAAGACAGGGTAGGTG |
65 | 187 | NM_001030693.1 |
GAPDH = glyceraldehyde 3 phophate dehydrogenase; MUC2 = mucin 2; IgA = immunoglobulin A; ZO1 = zonula occludens-1; ZO2 = zonula occludens-2; CLDN1 = claudin-1; CLDN5 = claudin-5; CLDN = occludin; NFKB1 = nuclear factor kappa B subunit 1; TLR2B = toll-like receptor 2 family member B; TLR4 = toll-like receptor 4.