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Conjugation efficiencies of replicons with NSCV ori2 replication origins.
| Replicon† | Origin of replication | Conjugation‡ efficiency |
|---|---|---|
| pMA568 | N16961ori2 | 5.5 × 10-3 |
| pMA739 | NSCV1 ori2 | 1.7 × 10-2 |
| pMA755 | NSCV2 ori2 | 4.3 × 10-2 |
| pMA899 | F plasmid origin | 3.2 × 10-2 |
| pMA135 | oriR6K only | 0 |
†All replicons possess an oriR6K and conjugated from E. coli strain WM3064 to MG1655. ‡Efficiency of one representative experiment is given as ratio of conjugant CFU over total recipient CFU.
