FIGURE 7.
Schematic presentation (A) and transcript levels (B, n = 3) of genes involved in leaf sulfur metabolism. Left two tiles of each gene represent the log2- fold change (FC) between the bou-2 mutant and the WT plants at both day (D) and night (N) with false color code ranging from red (higher in bou-2) to blue (higher in WT). Right two tiles represent the log2-fold change (FC) between day and night within the WT plants and the bou-2 mutant with false color code ranging from green (higher at night) to yellow (higher at day). Asterisks indicate differential gene expression as determined by Sleuth (∗FDR < 0.05, ∗∗FDR < 0.01, and ∗∗∗FDR < 0.001). APS, adenosine 5′- phosphosulfate; Cys, cysteine; Cyst, cystathionine; Glu, glutamate; γ-GluCys, γ-glutamylcysteine; GSH, glutathione; GS-X, glutathione conjugate; Hcy, homocysteine; Met, methionine; OAS, O-acetylserine; OPH, O-phosphohomoserine; PAPS, 3′-phosphoadenosine 5′- phosphosulfate; ROH, hydroxylated precursor; SAH, S-adenosylhomocysteine; SAM, S-adenosylmethionine; Ser, serine; Thr, threonine; X-CysGly, cysteinylglycine conjugate. Enzymes: ATPS, ATP sulfurylase; APK, APS kinase; SOT, sulfotransferase; APR, APS reductase; SIR, sulfite reductase; SAT, serine acetyltransferase; OAS-TL, OAS(thiol)lyase; CGS, cystathionine gamma-synthase; TS, threonine synthase; CBL, cystathionine beta-lyase; MS, methionine synthase; SAM, S-adenosylmethionine synthetase; ECS, gamma -glutamylcysteine synthetase; GSHS, glutathione synthetase; GST, glutathione-S-transferase; GGT, gamma-glutamyltransferase.