. 2018 Nov 27;9:1709. doi: 10.3389/fpls.2018.01709

Table 1.

Metabolite contents present in arbitrary units (arb.u. mg^-1 FW) in leaves of Arabidopsis thaliana WT and the bou-2 mutant at day and night.

	Metabolites	Day		Night

		Col-0	BOU	Col-0	BOU
		Mean SD	Mean SD	Mean SD	Mean SD
Sugars	Fructose	2099.02 ± 251.05a*	3128.45 ± 830.72*	1301.38 ± 229.22^a	7401.39 ± 1774.24^b
	Glucose	3587.00 ± 1178.37	2804.70 ± 428.45**	2879.78 ± 400.50^a	8374.86 ± 1683.53^b
	Maltose	189.11 ± 67.26***	108.42 ± 10.53 ***	648.76 ± 13.94^a	267.89 ± 11.22^b
	Manose	45.86 ± 11.28	34.38 ± 2.27*	61.51 ± 4.59^a	46.14 ± 5.28^b
	Raffinose	152.52 ± 19.61^a	0.37 ± 0.30^b	237.985 ± 86.18^a	0.78 ± 0.74^b
	Sorbitol	43.79 ± 9.81^a**	29.55 ± 3.92^b***	17.95 ± 2.31^a	11.59 ± 0.48^b
	Sucrose	27661.97 ± 3859.03	25636.56 ± 1699.43*	28159.45 ± 1150.18^a	19927.22 ± 2573.64^b
	Xylose	88.33 ± 9.402	94.13 ± 10.99	92.26 ± 1.66	99.25 ± 7.33
Acids involved in TCA	Alpha-ketoglutarate	24.95 ± 9.46^a	304.22 ± 56.91^b*	19.49 ± 0.90^a	200.92 ± 21.30^b
	Fumaric acid	56114.14 ± 3677.00	40858.41 ± 9527.63	54647.13 ± 5059.05	51553.88 ± 2507.11
	(Iso)citric acid	1596.74 ± 664.04	1429.45 ± 387.71*	949.93 ± 272.408	580.83 ± 357.611
	Malic acid	4412.55 ± 651.93	4701.32 ± 546.39*	4509.1 ± 451.8^a	6160.18 ± 297.17^b
	Proline	104.85 ± 20.13*	212.29 ± 64.24	57.74 ± 2.38	111.34 ± 36.35
	Succinic acid	349.30 ± 55.03^a	625.65 ± 148.07^b*	436.55 ± 20.61^a	901.08 ± 24.12^b
Secondary metabolites	Glycolic acid	31.38 ± 2.88^a**	40.04 ± 2.23^b***	19.96 ± 0.62^a	22.88 ± 0.28^b
	Shikimate	340.53 ± 6.73^a*	192.09 ± 22.094^b*	411.33 ± 26.960^a	267.06 ± 37.963^b
	Quinic acid	1.75 ± 0.17**	1.51 ± 0.072***	2.54 ± 0.09^a	2.86 ± 0.03^b
Amino acids	Alfa alanine	1212.82 ± 203.49^a**	1910.62 ± 36.03^b**	564.43 ± 30.08^a	5831.68 ± 1123.55^b
	Aspartic acid	20.54 ± 3.24^a*	8.31 ± 0.39^b***	12.22 ± 2.33^a	19.57 ± 2.26^b
	Beta-alanine	34.30 ± 0.68^a	673.08 ± 98.08^b	35.54 ± 2.31^a	632.54 ± 100.52^b
	GABA	2774.76 ± 99.02^a*	4649.08 ± 367.88^b	2331.90 ± 182.87^a	4597.98 ± 752.32^b
	Glyceric acid	153.36 ± 32.22^a*	310.06 ± 21.39^b***	90.48 ± 8.24^a	133.94 ± 5.85^b
	Glycine	637.59 ± 192.41^a	338399.99 ± 8441.71^b	423.50 ± 157.21^a	325863.04 ± 11640.42^b
	(Iso)leucine	11.32 ± 0.88^a	78.62 ± 25.44^b*	12.00 ± 1.97^a	16.75 ± 0.53^b
	Leucine	11.24 ± 0.24^a	73.48 ± 16.99^b**	12.97 ± 2.36	15.84 ± 1.61
	Serine	13.01 ± 0.33^a***	52.78 ± 4.64^b	6.28 ± 0.76^a	63.91 ± 7.93^b
	Threonine	7.52 ± 1.09^a***	41.38 ± 8.22^b*	2.01 ± 0.38^a	16.17 ± 5.34^b
	Valine	356.05 ± 101.33^a**	1169.77 ± 19.021^b***	631.58 ± 8.99^a	717.51 ± 37.84^b
Sugar alcohols	Methionine	6.374 ± 0.761^a	3.055 ± 0.679^b	4.950 ± 1.162^a	1.797 ± 0.955^b
	Glycerol	1728.74 ± 83.86^a	2065.05 ± 40.31^b*	1816.16 ± 111.70^a	3055.49 ± 415.78^b
Organic acids	Mannitol	98.69 ± 15.51	88.92 ± 5.43***	70.20 ± 17.21	42.33 ± 7.98
	Gluconic acid	5.89 ± 0.34^a*	9.01 ± 1.77^b	4.39 ± 0.63	5.84 ± 0.95
	Hydroxyglutarat	28.58 ± 6.03^a	55.15 ± 1.38^b***	40.25 ± 7.89	36.88 ± 1.94
	Maleic acid	57.91 ± 11.28	51.36 ± 7.60	54.06 ± 4.93	48.72 ± 7.45
	Malonic acid	0.86 ± 0.17^a***	3.85 ± 0.88^b	2.61 ± 0.14	3.88 ± 0.78
	Myoinositol	2633.09 ± 246.98^a**	1085.52 ± 120.82^b**	4197.10 ± 364.20^a	1742.63 ± 211.04^b

Plants were grown at elevated (3000 ppm) CO₂ and harvested after 24 h of ambient CO₂ level ca. 10 min after light onset and 3 h before light onset. Small letters indicate significant differences between different plant types within same treatment (P < 0.05). Asterisks indicate significant differences between day and night within same plant type (^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001). All values are means ± standard deviation of 3 replicates.