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. Author manuscript; available in PMC: 2018 Dec 7.
Published in final edited form as: Cell Rep. 2018 Oct 16;25(3):598–610.e5. doi: 10.1016/j.celrep.2018.09.072

Figure 3. Genome Stability of Epithelial Cells Expanded with the EpiX Medium.

Figure 3.

(A) HFKs, HBECs, and PrECs expanded with the EpiX medium over 40 PDs retained diploid karyotypes.

(B) EpiX-expanded HFKs did not form tumors after 12 weeks in female nude mice (1 × 107 cells per mouse, n = 6 in each group). Data are represented as mean ±SD, n = 6.

(C) Data analysis workflow for whole-genome sequencing.

(D) Distributions of de novo SNVs identified by whole genome-sequencing in late-passage HFK and CF samples. Venn diagram showed the overlap between the CF and HFK samples.

(E) Genes that were affected by missense de novo SNV (all heterozygous) after 40 PDs expansion in the EpiX medium. Two separate missense SNVs were found in the DDX11 gene in the CF and HFK samples.