Fig. 4.

Characterization of Cr2c-Cyclop1 activity under different conditions. a Dynamic activity of Cr2c-Cyclop1. All three samples were under initial dark stage for 30 s and then put to constant dark (blue square), constant green light illumination (532 nm, ~ 20 μW/mm², green rhombus), and 3-min green light followed by constant dark (gray dot). Samples were collected and measured at different time points indicated in the figure. n = 3, error bars = SD. b Cr2c-Cyclop1 activity influenced by ATP, Mg²⁺, and Ca²⁺. Mg²⁺ were depleted (no MgCl₂), replaced by Ca²⁺ (5 mM CaCl₂) or ATP depleted (no ATP) from the standard reaction buffer. n = 4, error bars = SD. c Cr2c-Cyclop1 activity at different temperatures (10, 20, 30, and 40 °C). n = 3, error bars = SD. d Cr2c-Cyclop1 activity at different pHs (6.3, 7.3, 8.3). n = 3, error bars = SD. Illumination conditions were same for a–d