Hepatic deletion of miR-221/222 reduces liver fibrosis in NASH mice. (a and b) Representative Sirius red staining (a) and Masson staining (b) of the liver sections from miR-221/222-LKO and control mice treated with MCDD or control diet (upper part) and CCl4 or vehicle (bottom part). Scale bars, 100 μm. (c and d) Analysis of the Sirius red-positive area (c) and Masson-positive area (d) of the liver sections from miR-221/222-LKO and control mice treated with MCDD or control diet (upper part) and CCl4 or vehicle (bottom part) (n = 4–5). (e) Hepatic hydroxyproline concentration of miR-221/222-LKO and control mice treated with MCDD or control diet (upper part) and CCl4 or vehicle (bottom part) (n = 3–5). (f) TEM micrographs (arrowheads, collagen) of liver samples from miR-221/222-LKO and control mice treated with MCDD or control diet. Scale bars, 5 μm. Data represent mean ± SD. *P < .05, **P < .01, ***P < .001, one-way ANOVA.