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Effect of BRIT1 siRNA-2 on the viability of HTR8/SVneo cells. HTR8/SVneo cells were transfected with PBS (control), unspecific scrambled siRNA (control siRNA), BRIT1 siRNA-1, and BRIT1 siRNA-2 plasmids. (A) RT-qPCR was performed to test the mRNA level of BRIT1. (B) Western blotting was used to assess the protein level of BRIT1. (C) Cell viability was analyzed by CCK-8 analysis. * P<0.05, ** P<0.01, versus control; # P<0.05, ## P<0.01, versus control si-RNA.
