Effect of BRIT1 siRNA-2 on invasion-related factors in HTR8/SVneo cells. (A) The activities of pro-MMP-2 and pro-MMP-9 were investigated by gelatin zymography assay. (B–E) The mRNA levels of MMP-2 (B), MMP-9 (C), TIMP-1 (D), and TIMP-2 (E) were surveyed using RT-qPCR. (F, G) The proteins levels of MMP-2, MMP-9, TIMP-1, and TIMP-2 were identified by Western blotting. * P<0.05, ** P<0.01, versus control; #
P<0.05, ##
P<0.01, versus control siRNA.