Fig. 2.

Hyper expression of S1PR1 in gastric cancer cells identified drug resistance.

(A) Transcript levels determined by QPCR and (B) protein expression levels determined by Western blot of indicated genes of SGC7901 and SGC7901/DDP gastric cancer cell lines. Data were expressed as mean ± SD, n = 5. *P < 0.05 (Student's t-test). (C) Immunofluorescence double staining of S1PR1 (green) and pY-STAT3 (red) in SGC7901 and SGC7901/DDP cells. White arrow indicated the colocalization of each protein. DAPI(blue) was adopted to reveal the nuclear of the cells. Scale bar: 20 μM. (D) Transcript levels and (E) protein expression levels of indicated genes of different gastric tumor cell lines were detected by QPCR or Western blot, respectively. Data were expressed as mean ± SD, n = 5. (F) Transcript levels of S1PR1 in gastric cancer patients who were reported to receive chemotherapy drug in TCGA database. The patients were divided into two groups of PD (progressive disease) and CR (complete remission/response) according to the follow up report.