Fig. 7.

Anti IL-20Rb neutralizing antibody reduces the expression of IL-20 cytokines and their receptors in the lung of Streptococcus pneumoniae -infected mice. mRNA levels of the IL-20 related cytokines (IL-19, IL-20, IL-22, IL-24) and their receptors (IL-20Ra, IL-20Rb and IL-22Ra) in lung tissue of mice infected with Sp evaluated by RT-qPCR. Results were expressed as fold increase compared to the mice exposed to PBS and using expression of GAPDH as a housekeeping gene. Results were expressed as mean ± SEM and statistical analyses were performed by Mann-Whitney U analysis and non-parametric Kruskal-Wallis followed by Dunns posttest in comparison with PBS controls, or with Sp + IgG. *P < .05, **P < .01 and ***P < .001. Three independent experiments have been performed with 4–5 mice in each group.