Fig. 3.

Mildly elevated urate in UOx cKO mice is not associated with BP changes. (a) HPLC analysis of serum urate levels in UOx cKO mice and ‘WT’ (floxed UOx with no cre). (b) Invasive SBP versus serum urate of UOx cKO mice and ‘WT’ at baseline (‘WT’: r²=0·138, p=0·36; cKO: r²=0·193, p=0·20; pooled: r²=0·163, p=0·09). Invasive SBP (c) and HR (d) measurement before (baseline) and after vehicle administration. n=9 and 8, male four-month old UOx cKO and littermate ‘WT’ mice. (e) Noninvasive SBP measurement before and two weeks after completion of tamoxifen injection, during three weeks of high salt diet, low salt diet and two weeks of washout, and pre-, post (15 min) each L-NAME administration. The second L-NAME administration was seven days after first L-NAME administration. Asterisks for significant change comparing preceding time point from different treatment are above the plot for WT and below the plot for UOx cKO. n=16 and 14, UOx cKO and littermate WT mice, all female, average three-month old at the baseline. *p<0·05, **p<0·01, ***p<0·001.