Figure 4.

Interindividual variations of selected metabolites and metabolite ratios regarding the study cohort. (A) Average normalized concentrations of amino acids have been calculated for each patient, and are displayed in a proportional bar chart to allow comparison of the individual subjects at a glance (colors represent single amino acids, as indicated by the legend). (B) Glu/Gln was calculated of absolute amino acid concentrations. The resulting values are shown as box blots for each subject numbered 1 to 12. ANOVA was computed and revealed significant statistical differences within the cohort (data not shown). (C) A surrogate parameter for glutaminolysis, namely the ratio of the sum of amino acid concentrations of aspartate, alanine, and glutamate to the concentration of glutamine was calculated. The resulting values are shown as box blots for each subject (numbers 1–12). ANOVA was computed and revealed significant statistical differences within the cohort (q < 0.001, data not shown). Values of subjects 5 and 10 are significantly different from the rest as revealed by Student's t-test.