Figure 1. Generation of E44 chimeric porcine fetus in vivo by complementation of MITF ^L247S/L247S embryos with donor blastomeres derived from LW PEFs.

• A
  Four fetuses (named NW‐1, NW‐2, NW‐3, and NW‐4) with different eye morphology were retrieved at E44 by Caesarean section. The arrows identify the melanin in the eye.
• B
  DraI digestion of PCR products from the multiple organs of the four fetuses (top). NC, negative control with no genomic DNA loaded. Mut, MITF ^L247S/L247S genomic DNA loaded. WT, LW genomic DNA loaded. Multiple organs of the four fetuses were examined for KIT expression by agarose gel electrophoresis (bottom). Mut, MITF ^L247S/L247S genomic DNA loaded. WT, LW genomic DNA loaded.

Source data are available online for this figure.