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. 2018 Nov 16;10(12):e8861. doi: 10.15252/emmm.201808861

Figure EV2. The chimeric contributions in vitro were detected by immunofluorescence and RFLP analysis simultaneously.

Figure EV2

  • A
    Representative immunofluorescence images show the expression of RFP and GFP simultaneously in chimeric embryos. GFP‐labeled and RFP‐labeled blastocysts are shown as negative controls. Scale bar, 50 μm.
  • B
    Representative immunofluorescence images showed the expression of GFP in the chimeric blastocyst. Scale bar, 50 μm.
  • C
    Genotyping for porcine blastocysts derived from injected embryos. Porcine MITF‐specific primers were used for the detection of chimeric contribution. Restriction enzymes DraI was used for digestion of PCR products. NC, negative control with no genomic DNA loaded. Mut, Bama MITF L247S/L247S blastocyst DNA loaded. WT, Bama wild‐type blastocyst DNA loaded. B1‐B10, collected the injected blastocysts DNA loaded.