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. 2018 Nov 16;10(12):e8861. doi: 10.15252/emmm.201808861

Figure EV3. Genotyping for the chimeric contributions in fetuses and full‐term chimeric pig.

Figure EV3

  • A
    PCR amplification of MITF in the blastocysts. Mut, Bama MITF L247S/L247S blastocyst DNA loaded. WT1‐WT2, DNA of Bama WT number 1 and 2 blastocysts loaded. B1‐B10, DNA of the injected blastocysts from number 1 to number 10 loaded.
  • B
    PCR amplification of MITF in the E44 fetuses. NC, negative control with no genomic DNA loaded. Mut, MITF L247S/L247S genomic DNA loaded. WT, LW genomic DNA loaded.
  • C
    PCR amplification of MITF and PCR‐RFLP analysis of MITF in E60 fetuses. NC, negative control with no genomic DNA loaded. Mut, MITF L247S/L247S genomic DNA loaded. WT, Bama genomic DNA loaded. NW‐5‐NW‐11, fetuses at E60.
  • D
    PCR amplification of MITF and digestion of the PCR products in the multiple organs of the NW‐7 and NW‐8 fetuses. NC, negative control with no genomic DNA loaded. Mut, MITF L247S/L247S genomic DNA loaded. WT, Bama genomic DNA loaded.
  • E
    PCR amplification of MITF and PCR‐RFLP analysis of MITF in the full‐term chimeric pig. NC, negative control with no genomic DNA loaded. Mut, MITF L247S/L247S genomic DNA loaded. WT, Bama GFP‐labeled genomic DNA loaded.
  • F
    PCR amplification of MITF in multiple organs of the full‐term chimeric pig. NC, negative control with no genomic DNA loaded. Mut, MITF L247S/L247S genomic DNA loaded. WT, Bama GFP‐labeled genomic DNA loaded.