Figure 3.

NOCT orthologs share a conserved EEP domain but have divergent N-termini. A) Amino acid sequence alignment of NOCT orthologs from various model organisms. Sequences were aligned and colored as in Fig. 2; darker shading indicates greater functional group similarity (charge, hydrophobicity, etc.). Strictly conserved positions are in white text with black background. Putative hNOCT catalytic (*) and basic patch residues (inverted triangles) are indicated. The putative PPARγ-interacting peptide is highlighted by a yellow bar. Uniprot accession numbers: D. melanogaster A8JQX3, H. sapiens Q9UK39, R. norvegicus Q9ET55, M. musculus O35710, X. laevis P79942, X. tropicalis Q28CV0, D. rerio NOCTB A0A0G2KRI0, D. rerio NOCTA E7F177. B) Pair-wise amino acid identities among NOCT orthologs.