Table 3.
Overexpression of B. subtilis tRNALysUUU and tRNAGlnUUG in E. coli mnmC(o) and mnmC(m) mutant strains.
| E. coli mutant straina | Nucleoside distribution (%)b in: |
|||||
|---|---|---|---|---|---|---|
| Bs-tRNALys |
Bs-tRNAGln |
|||||
| cmnm5s2U | nm5s2U | mnm5s2U | cmnm5s2U | nm5s2U | mnm5s2U | |
| ΔmnmC(o) | 56 | 0 | 44 | 76 | 0 | 24 |
| mnmC(m)-G68D | 0 | 100 | 0 | 36 | 64 | 0 |
| mnmC-W131stop | 65 | 35 | 0 | 90 | 10 | 0 |
aThe E. coli strains were IC6629 [ΔmnmC(o)], IC6018 [mnmC(m)-G68D] and IC6019 [ΔmnmC-W131stop].
bSpecific Bs-tRNAs were purified from the indicated E. coli strains and HPLC analyzed. The nucleoside distribution (%) was calculated as in Table 2. Each value is the mean of at least three independent experiments. Standard deviations were within ± 10%.