Figure 3.

rbm-5a gene structure, RBM-5A protein domains and positions of rbm-5 mutations.

(a) Exon-intron structures of the rbm-5a isoform (designed using the Exon-Intron Graphic Maker software at www.wormweb.org according to gene sequences at www.wormbase.org). Exonic regions targeted by the CRISPR/Cas9 method are labeled on top as red bars. n5132 and n5119 mutations are indicated. The conserved RRM RNP1 sequence is shown, in which the F271I mutation corresponds to the second [FY] residue [43,44]. mac418 was obtained using sgRNA1, mac465, mac466 and mac467 were obtained using sgRNA2. mac468 was obtained using sgRNA2 and sgRNA3. (b) RBM-5 protein domains and positions of the n5119 missense mutation.