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. 2018 Oct 13;15(10):1295–1308. doi: 10.1080/15476286.2018.1526540

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — Fluorescent pictures of animals expressing a Prbm-5::GFP transcriptional fusion transgene and a Peft-3::rbm-5a cDNA::GFP translational fusion transgene.

(a) A low resolution picture of Prbm-5::GFP transgenic animals showing GFP expression in the anterior, posterior and ventral nerve cord (VNC). High resolution picture of the anterior (b), mid body (c) and posterior (d) of a Prbm-5::GFP transgenic animal. Low (e) and high (f) resolution pictures of a Peft-3::rbm-5a cDNA::GFP transgenic animal showing that the RBM-5A::GFP fusion protein was exclusively localized in the nuclei. We determined the identities of Prbm-5::GFP-positive cells by examining their anatomical positions, morphologies, patterns and neighboring cells based on the descriptions at www.wormatlas.org.