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. 2018 Oct 19;10(8):1214–1225. doi: 10.1080/19420862.2018.1521131

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© 2018 The Author(s). Published by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 6. — Acidic low-abundance isoforms. XDC traces (15 ppm) are plotted for top 3 glycoforms in combination with (a) one sialic (neuraminic) acid (S1G0F or S1G1F)-containing N-glycan, (b) one sialic acid-containing N-glycan and one deamidation, or (c) two sialic acid-containing N-glycans. Abundance of each isoform is shown as a percentage relative to main-fraction adjusted XDC peak area of G0F/G0F. (d), (e), and (f) show mass spectra averaged across highlighted RT respectively in (a), (b), and (c).