Figure 4.

Luteolin inhibits proliferation of GBM organoids. Patient derived GBM528 or CCF1914 glioblastoma specimens were grown as 3-dimensional organoids for > 2 months to establish mature GBM tissue structures. Organoids were then treated with DMSO (control) or 30μM luteolin for 7 days. Treated organoids were probed for the mitotic marker phospho-Histone H3 by immunohistochemistry. Full digital slide scans were performed and 3–6 non-overlapping high power fields were extracted from each sample for automated quantification. Representative full 20x microscope fields are also shown. Statistical significance was calculated by one-way ANOVA and t test. All data are shown as means ± s.d. and performed as technical triplicates. (*P < 0.05, **P < 0.005).