Table 2.

Class-II preQ₁ riboswitches – Thermodynamic and kinetic parameters of ligand binding and cellular activity.

Ligand No.	KD [nM]	kobs [s−1]	koff [s−[1]] *	IC50 [µM] **
1	430	1.07	0.084733	96 (65%)
2	2830	> 10***	n.d.	n.d.
3	97	1.05	0.019982	> 500
4	148	0.80	0.022999	> 1000
5	254	0.70	0.033841	71 (80%)
6	432	0.68	0.054080	40 (80%)
7	203	0.75	0.029262	92 (68%)
8	4580	0.72	0.031532	116 (65%)
9	1030	1.06	0.181061	328 (53%)

* off-rates were calculated from k_off = k_obs/(1+([L]/K_D)) (ref. [51]);

** number in brackets represents percentage of repression at saturating ligand concentration;

*** estimated value;

n.d. not determined