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Illustration of aldehyde tag scanning insertion.
In order to carry out an unbiased scan of aldehyde tag placement throughout the antibody constant regions, we designed a scheme in which the FGE recognition sequence, LCTPSR, would be inserted next to each amino acid residue in the constant regions of the light and heavy chains. Our numbering system is sequential based on the first N-terminal residue in the constant region of each antibody chain.
