Table 1.

Summary of results from aldehyde tag scanning insertions across 436 sites in antibody constant regions.

Antibody domain	Number of Sites Scanned	% Scanned Sites Expressed as mAbsa	% of Expressed mAbs Conjugatedb	% of High DAR Conjugatesc	% of Highly Monomeric High DAR ADCsd	Total Number of Manufacturable Sitese	% of Manufacturable Sites Per Sites Scanned
Light chain constant region	106	75	50	22	88	14	13
Heavy chain CH1	98	99	70	46	51	23	23
Heavy chain Hinge	15	100	87	40	0	0	0
Heavy chain CH2	110	57	60	35	27	6	5
Heavy chain CH3	107	80	72	37	44	14	13
All constant regions (light and heavy chain)	436	76	68	37	49	58	13

^aTiters ≥ 10 mg/L (light chain) or ≥ 80 mg/L (heavy chain) and purifiable by Protein A.

^bAnalyzable ADCs with DARs ≥ 0.5.

^cADCs with DARs above 1.3 (light chain) or 1.5 (heavy chain)

^dOf High DAR conjugated antibodies, % monomer above 95.

^eNumber of highly monomeric, high DAR ADCs produced per number of sites scanned.