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. 2018 Oct 2;10(8):1200–1213. doi: 10.1080/19420862.2018.1512328

Figure 12.

Figure 12.

MALDI-MS spectrum (5–10 kDa region) of the hinge peptide fraction from the LC-MALDI dataset of the IgG2 B isoform, obtained from an sDHB matrix preparation. Within this fraction were approximately 40 variants of hinge peptides from all three IgG2 disulfide isoforms. Color-coded IgG2-A (blue), -A/B (red), and -B (green) peaks provide an overview of the isoform-specific hinge peptide fragments in a heterogeneous mixture. “mc” = number of missed cleavage chains in peptides.