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SEC separation of Bis-A under different conditions on an Aquity BEH SEC-200 column. Shown are SEC separations on (A) 150 × 4.6–mm column with150 mM sodium phosphate, pH 6.8, mobile phase; (B) 150 × 4.6–mm column with 750 mM NaCl, 150 mM sodium phosphate, pH 6.8, mobile phase; and (C) 300 × 4.6–mm column with 750 mM NaCl, 150 mM sodium phosphate, pH 6.8, mobile phase.
