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A) DSC thermograms of the trastuzumab constructs. B) Trastuzumab Fabs variants binding to HER2 by BLI. Binding experiments were performed on an OctetRED. HER2-hFc was bound at 10 µg/mL to anti-human IgG biosensors. Each Fab was tested at 10 nM. All constructs exhibit HER2 binding. C) Table of Tms of trastuzumab Fab variants by DSC. D) Select DSC thermograms of trastuzumab Fabs.
