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. 2018 May 1;29(3):283–291. doi: 10.5152/tjg.2018.17484

Table 1.

Primers used for the determination of SNP rs4986790 and SNP rs3804099 in the TLR2 and TLR4 genes, respectively

PCR products Primer Gene
TLR2 rs3804099 Forward outer: 5-ATTGCAAATCCTGAGAGTGGGAA-3 Common product size: 349 bp
Reverse outer: 5-CAAACTTTCATCGGTGATTTTCACA-3 TT: 228 bp
Forward inner(T allele): 5-CCAAAAAGTTTGAAGTCAATTCAGAAT-3 CC:173 bp
Reverse inner(C allele): 5-TCATATGAAGGATCAGATGACTTCCG-3 CT: 228 bp and 173 bp
TLR4 rs4986790 Forward outer: 5′-TGAACCCTATGAACTTTATCC-3′ Common product size: 383 bp
Reverse outer: 5′-GTTAACTAATTCTAAATGTTGCCATC-3′ AA: 147 bp
Forward inner(A allele): 5′-GCATACTTAGACTACTACCTCGATGA-3′ GG:287 bp
Reverse inner(G allele): 5′-CAAACAATTAAATAAGTCAATAATAC-3′ AG:147 bp and 287 bp

PCR: Polymerase chain reaction; SNP: Single nucleotide polymorphism; TLR: Toll-like receptors