Table 1.
PCR products | Primer | Gene |
---|---|---|
TLR2 rs3804099 | Forward outer: 5-ATTGCAAATCCTGAGAGTGGGAA-3 | Common product size: 349 bp |
Reverse outer: 5-CAAACTTTCATCGGTGATTTTCACA-3 | TT: 228 bp | |
Forward inner(T allele): 5-CCAAAAAGTTTGAAGTCAATTCAGAAT-3 | CC:173 bp | |
Reverse inner(C allele): 5-TCATATGAAGGATCAGATGACTTCCG-3 | CT: 228 bp and 173 bp | |
TLR4 rs4986790 | Forward outer: 5′-TGAACCCTATGAACTTTATCC-3′ | Common product size: 383 bp |
Reverse outer: 5′-GTTAACTAATTCTAAATGTTGCCATC-3′ | AA: 147 bp | |
Forward inner(A allele): 5′-GCATACTTAGACTACTACCTCGATGA-3′ | GG:287 bp | |
Reverse inner(G allele): 5′-CAAACAATTAAATAAGTCAATAATAC-3′ | AG:147 bp and 287 bp |
PCR: Polymerase chain reaction; SNP: Single nucleotide polymorphism; TLR: Toll-like receptors