Detection of HPV DNA using MY09/11 primers
M, 100 bp DNA ladder; Neg, negative control with sterilized water as template; HPV16 and HPV18, positive controls with HPV16/pBR322 and HPV18/pBR322 templates; lanes 1–7 were the results of HPV DNA detected from different esophageal carcinoma samples
B. Detection of HPV 16 using HPV16 E6 specific primers
M, 100 bp DNA ladder; Neg, negative control with sterilized water as template; HPV16, positive control with HPV16/pBR322 template; HPV18, specific control for HPV16 using HPV18/pBR322 DNA template; lanes 1–7 were the results of HPV16 detected from different esophageal carcinoma samples