Figure 1. Western blot showing expression of ERβ2 and ERβ5 where the first lane is control transfected (empty transposon system without variant cDNA), then ERβ2 or ERβ5 stably transfected PC3 cells in lanes 2 and 3.

30 µg of protein was separated on SDS-PAGE and the variants were detected using 14C8 N-terminal antibody which is detecting pan ERβ (all variants). All western blots are performed in the absence of doxycycline with expression of the variants at maximum level.