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. 2018 Nov 20;9(91):36256–36272. doi: 10.18632/oncotarget.26332

Figure 1. CR overexpression promotes migration and invasion in vitro.

Figure 1

(A) Western Blot analysis demonstrating lentiviral-mediated CR overexpression in SPC111 and MSTO-211H cells. GAPDH was used as loading control. (B) Comparison of wound closure kinetics between wt (blue) and CR- overexpressing cells (green) shown for SPC111 and MSTO-211H cells monitored with the IncuCyte™ imaging system every 2 h. A faster closing of the scratch is observed for CR-overexpressing cells when compared with wt cells. (C) Invasiveness determined by a modified ‘wound healing assay’ with an added matrix barrier component (Matrigel™). Comparison of wound closure kinetics between wt (blue) and CR-overexpressing cells (red). CR-overexpressing SPC111 and MSTO-211H cells completely closed the wound after 90 h and 60 h, respectively. Neither of the wt cells were capable of closing the gap during the observation period (140 h; n = 4 independent experiments). (D) Representative examples of time-lapse images taken after confluent monolayers of SPC111 and MSTO-211H (wt and CR-overexpressing cells) were scratched (time point 0 h), and after 90 h and 60 h (post-scratch), respectively. Scale bar: 400 μm.