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. 2018 Dec 4;9(6):1103–1121. doi: 10.14336/AD.2018.1110

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Copyright: © 2018 Junfen et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — miR-1292 inhibits osteoblast differentiation. (A) The expression of osteoblast-related genes was analyzed by qRT-PCR on day 6 of hADSC osteogenic differentiation. (B) Western blot analysis of protein levels of osteogenic markers on day 6. (C) ALP staining and ALP activity were assessed on day 4 of osteoblast differentiation. (D) Alizarin red staining was performed to indicate mineral deposition on day 15. (E) qRT-PCR was performed to analyze the expression of osteoblast marker genes on day 6. (F) Western blot analysis of the protein levels of osteogenic markers on day 6. (G) ALP staining and ALP activity were assessed on day 4 of osteogenic differentiation. (H) Alizarin red staining was performed to indicate mineral deposition on day 15. GAPDH was used as an internal control for western blot analysis. qRT-PCR data were normalized to GAPDH expression, results are presented as the mean ± SD, n = 3. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared to the control. Scale bars: 200 μm.