Figure 7.

FZD4 knockdown reverses the effect of miR-1292 inhibition on cellular senescence and osteogenic differentiation of hADSCs. (A) SA-β-gal staining was performed to detect SA-β-gal secretion. (B) qRT-PCR was used to analyze senescence-associated and osteogenic markers expression after different treatments. (C) Western blot was performed to analyze the expression of cellular senescence and osteogenic factors. (D and E) ALP staining and ALP activity assays were performed on day 4 of osteogenic differentiation. (F) Alizarin red staining was performed to indicate calcification on day 12 of osteogenic differentiation. GAPDH was used as an internal control for western blot analysis, whereas qRT-PCR data were normalized to PPIA or GAPDH; results are presented as the mean ± SD, n = 3. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared to the control. Scale bars: 200 μm.