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. Author manuscript; available in PMC: 2018 Dec 7.
Published in final edited form as: Methods Enzymol. 2010;485:147–160. doi: 10.1016/B978-0-12-381296-4.00009-9

Figure 9.1.

Figure 9.1

Constitutive signaling activities of TRH-R2 and TRH-R1 measured by reporter gene assay in HEK 293 EM cells. HEK 293 EM cells were transfected with increasing amounts of receptor expression plasmids and a single amount of reporter gene plasmid. After 24 h, luciferase activities (relative light units, RLU) and maximum specific ligand binding (receptor density, Bmax) were measured. The figure is adapted from Wang and Gershengorn (1999)