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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Sens Actuators B Chem. 2018 Oct 4;279:447–457. doi: 10.1016/j.snb.2018.09.121

Figure 4. Characterization of the MERCA for miRNA detection.

Figure 4.

(A) Specificity tests using let-7a, let-7b, let-7c, miR21, miR200a and miR200b. The concentration of each miRNA was 5.0×10−11 M. F: fluorescence intensity for miRNA detection; F0: background signal obtained with buffer. Inset: representative fluorescence images obtained with the buffer blank (top) and the let-7a sample (bottom), respectively. Scale bar: 250 μm. (B) Semi-log calibration plot of the MERCA assay for detection of a serial dilutions of let-7a in the range from 5.0×10−20 to 5.0×10−15 moles per reaction. (C) Semi-log calibration plot of the MERCA assay for detection of a serial dilutions of miR21 in the range of 5.0×10−20 to 5.0×10−17 moles per reaction. The linear curves were fitted by the least-squares fitting. The reactions were conducted with 10−8 M padlock probe under the optimal conditions. Error bars are the standard deviation from three replicates.