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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Sens Actuators B Chem. 2018 Oct 4;279:447–457. doi: 10.1016/j.snb.2018.09.121

Figure 5. Detection of miRNAs in total RNA and crude cell lysate using the MERCA and standard RT-qPCR.

Figure 5.

(A) Comparison between the standard RT-qPCR assay that requires multi-step sample preparation procedures and the MERCA for direct miRNA analysis with minimal sample treatment. (B) Calibration curves for the TaqMan RT-qPCR detection of synthetic let-7a and miR21 miRNAs, respectively. The linear curves were fitted by the leastsquares fitting. (C) Comparison of the concentrations of let-7a in the 0.1 μg/mL and 1 μg/mL MCF-7 total RNA samples measured by the RT-qPCR and the MERCA, respectively. (D)Comparing the MERCA and RT-qPCR analysis of miR-21 in the diluted samples of crude A549 whole cell lysate. 4.6 mg/mL A549 cell lysate was first diluted with the lysis buffer by 10 and 100 folds, respectively, prior to analysis. For RT-qPCR, total RNA was first extracted from the diluted A549 cell lysate samples, followed by RT and qPCR analysis. The concentrations of let-7a and miR-21 were calculated using the calibration curves in (B) for RTqPCR and in Fig. 4 for the MERCA. Error bars are one standard deviation (n = 3).