Table 1.
Name | Sequences |
---|---|
Padlock probea | 5′-phosphate-TCA CTA TGG TCC CTC AGC AGC ACA ATA AAT CAC TAT GGT CCC TCA GCA GCA CAA TAA ATC ACT ATG GTC CCT CAG CAG CAC AAT AAA-3′ |
let-7a capture probe | 5′-NH2-C12-AAA AAA AAA AAA AAC TAT ACA AC-3′ |
miR-21 capture probe | 5′-NH2-C12-AAA AAA AAA AAA TCA ACA TCA GT-3′ |
let-7a RCA adapterb | 5′-phosphate-CTA CTA CCT CA A AAA AAA AAA AAG ACC ATA GTG ATT TAT TGT GCT-3′ |
miR-21 RCA adapterb | 5′-phosphate-CTG ATA AGC TA AA AA AAA AAA AAG ACC ATA GTG ATT TAT TGT GCT-3′ |
let-7a | 5′-UGA GGU AGU AGG UUG UAU AGU U-3′ |
let-7bc | 5′-UGA GGU AGU AGG UUG UGU GGU U-3′ |
let-7cc | 5′-UGA GGU AGU AGG UUG UAU GGU U-3′ |
miR-21 | 5′-UAG CUU AUC AGA CUG AUG UUG A-3′ |
miR-200a | 5′-UAA CAC UGU CUG GUA ACG AUG U-3′ |
miR-200b | 5′-UAA UAC UGC CUG GUA AUG AUG A-3′ |
The bold letters show recognition sequences of nicking enzyme.
The italic letters are the sequences to hybridize with miRNAs.
The underlined bases show the different bases between let-7b or let-7c and let-7a.