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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Sens Actuators B Chem. 2018 Oct 4;279:447–457. doi: 10.1016/j.snb.2018.09.121

Table 1.

Sequences of oligonucleotides and miRNAs

Name Sequences
Padlock probea 5′-phosphate-TCA CTA TGG TCC CTC AGC AGC ACA ATA AAT CAC
TAT GGT CCC TCA GCA GCA CAA TAA ATC ACT ATG GTC CCT CAG CAG CAC AAT AAA-3′
let-7a capture probe 5′-NH2-C12-AAA AAA AAA AAA AAC TAT ACA AC-3′
miR-21 capture probe 5′-NH2-C12-AAA AAA AAA AAA TCA ACA TCA GT-3′
let-7a RCA adapterb 5′-phosphate-CTA CTA CCT CA A AAA AAA AAA AAG ACC ATA GTG ATT TAT TGT GCT-3′
miR-21 RCA adapterb 5′-phosphate-CTG ATA AGC TA AA AA AAA AAA AAG ACC ATA GTG ATT TAT TGT GCT-3′
let-7a 5′-UGA GGU AGU AGG UUG UAU AGU U-3′
let-7bc 5′-UGA GGU AGU AGG UUG UGU GGU U-3′
let-7cc 5′-UGA GGU AGU AGG UUG UAU GGU U-3′
miR-21 5′-UAG CUU AUC AGA CUG AUG UUG A-3′
miR-200a 5′-UAA CAC UGU CUG GUA ACG AUG U-3′
miR-200b 5′-UAA UAC UGC CUG GUA AUG AUG A-3′
a:

The bold letters show recognition sequences of nicking enzyme.

b:

The italic letters are the sequences to hybridize with miRNAs.

c:

The underlined bases show the different bases between let-7b or let-7c and let-7a.