KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal (C4) anti-β-actin | Santa Cruz Biotechnology | Cat# sc-47778 |
| Monoclonal (81G5) anti-PP2A subunit A | Cell Signaling Technology | Cat# 2041 |
| Monoclonal (52F8) anti-PP2A subunit C | Cell Signaling Technology | Cat# 2259 |
| Polyclonal anti-STAT5 | Cell Signaling Technology | Cat# 9363 |
| Monoclonal (D47E7) anti-p-STAT5 (Y694) | Cell Signaling Technology | Cat# 4322 |
| Polyclonal anti-Erk | Cell Signaling Technology | Cat# 9102 |
| Monoclonal (D13.14.4E) anti-p-Erk (T202/Y204) | Cell Signaling Technology | Cat# 4370 |
| Polyclonal anti-Akt | Cell Signaling Technology | Cat# 9272 |
| Monoclonal (D25E6) anti-p-Akt (T308) | Cell Signaling Technology | Cat# 13038 |
| Monoclonal (C29H4) anti-FoxO1 | Cell Signaling Technology | Cat# 2880 |
| Polyclonal anti-p-FoxO1 (T24) | Cell Signaling Technology | Cat# 9464 |
| Polyclonal anti-p-FoxO1 (S256) | Cell Signaling Technology | Cat# 9461 |
| Monoclonal (75D8) anti-FoxO3a | Cell Signaling Technology | Cat# 2497 |
| Monoclonal (D18H8) anti-p-FoxO3a (S253) | Cell Signaling Technology | Cat# 13129 |
| Monoclonal (2217) anti-S6 | Cell Signaling Technology | Cat# 2217 |
| Monoclonal (4856) anti-p-S6 (S235/236) | Cell Signaling Technology | Cat# 4856 |
| Polyclonal anti-p70 S6 kinase | Cell Signaling Technology | Cat# 9202 |
| Polyclonal anti-p-p70 S6 kinase (T389) | Cell Signaling Technology | Cat# 9205 |
| Polyclonal anti-PFKFB2 | Milipore | Cat# 07-1530 |
| Monoclonal (D4R1W) anti-p-PFKFB2 (S483) | Cell Signaling Technology | Cat# 13064 |
| Polyclonal anti-H2AX | Abcam | Cat# Ab10475 |
| Polyclonal anti-p-H2AX (S139) | Abcam | Cat# Ab2893 |
| Monoclonal (2524) anti-p53 | Cell Signaling Technology | Cat# 2524 |
| Monoclonal (C-2) anti-Bcl2 | Santa Cruz Biotechnology | Cat# sc-7382 |
| Polyclonal anti-p21 | Santa Cruz Biotechnology | Cat# sc-397 |
| Monoclonal (F-8) anti-p27 | Santa Cruz Biotechnology | Cat# sc-1641 |
| Polyclonal anti-p19 ARF (mouse) | Abcam | Cat# ab80 |
| Polyclonal anti-G6PD | Cell Signaling Technology | Cat# 8866 |
| Monoclonal (G-2) anti-PGD | Santa Cruz Biotechnology | Cat# sc-398977 |
| Polyclonal anti-TIGAR | Invitrogen | Cat# PA5-23053 |
| Polyclonal anti-Pax5 (for ChIP) | Santa Cruz Biotechnology | Cat# sc-1974 |
| Monoclonal anti-Pax5 (for western blot) | Cell Signaling Technology | Cat# 8970S |
| Monoclonal anti-Ikaros | Cell Signaling Technology | Cat# 14859S |
| Monoclonal anti-EBF1 | Abcam | Cat# ab108369 |
| Poly clonal anti-E2A (TCF3) | Santa Cruz Biotechnology | Cat# sc-349 |
| Monoclonal (1D3) anti-Mouse CD19 | BD Biosciences | Cat# 557655 |
| Monoclonal (RA3-6B2) anti-Mouse CD45R | Biolegend | Cat# 103224 |
| Monoclonal (M1/70) anti-Mouse CD11b | Biolegend | Cat# 101208 |
| Monoclonal (6C3) anti-Mouse Ly-51 (BP-1) | Biolegend | Cat# 108305 |
| Monoclonal (M1/69) anti-Mouse CD24 | BD Biosciences | Cat# 562477 |
| Monoclonal (11-26c.2a) anti-Mouse IgD | Biolegend | Cat# 405716 |
| Monoclonal (R6-60.2) anti-Mouse IgM | BD Biosciences | Cat# 550881 |
| Monoclonal (JC5-1) anti-Mouse Ig Light Chain, λ | Southern Biotech | Cat# 1175-09 |
| Monoclonal (187.1) anti-Mouse Ig Light Chain, κ | BD Biosciences | Cat# 562476 |
| Monoclonal (17A2) anti-Mouse CD3 | Biolegend | Cat# 555275 |
| Monoclonal (RM4-5) anti-Mouse CD4 | BD Biosciences | Cat# 553049 |
| Monoclonal (53-6.7) anti-Mouse CD8a | BD Biosciences | Cat# 553033 |
| Monoclonal (PK136) anti-Mouse NK1.1 | BD Biosciences | Cat# 553165 |
| Monoclonal (RB6-8C5) anti-Mouse Gr-1 (Ly-6G/Ly-6C) | BD Biosciences | Cat# 553128 |
| Monoclonal (TER-119) anti-Mouse TER-119 | BD Biosciences | Cat# 553673 |
| Monoclonal (2B8) anti-Mouse CD117 (c-Kit) | BD Biosciences | Cat# 553356 |
| Monoclonal (HIB19) anti-Human CD19 | BD Biosciences | Cat# 555413 |
| Biological Samples | ||
| Patient-derived pre-B ALL samples, see Table S2 | Müschen Laboratory | http://lymphoblasts.org/ |
| Patient-derived B-cell lymphoma samples, see Table S3 | Müschen Laboratory | http://lymphoblasts.org/ |
| Patient-derived B-cell lymphoma samples, see Table S3 | Weinstock Laboratory | https://www.proxe.org/ |
| Patient-derived chronic myeloid leukemia (CML) samples, see Table S4 | Thomas Ernst and Andreas Hochhaus | Jena University Hospital, Germany |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Imatinib | LC Laboratories | Cat# I-5577 |
| Idelalisib | Selleck Chemicals LLC | Cat# S2226 |
| Rapamycin | Sigma-Aldrich | Cat# 37094 |
| 4-hydroxy-tamoxifen | Sigma-Aldrich | Cat# 94873 |
| Doxycycline | Clontech Laboratories | Cat# 631311 |
| Tamoxifen | Sigma-Aldrich | Cat# 85256 |
| LB-100 | Selleck Chemicals LLC | Cat# S7537 |
| 6-AN | Sigma-Aldrich | Cat# A68203 |
| Critical Commercial Assays | ||
| Click-iT EdU Flow Cytometry Assay Kit | Life Technologies | Cat# C10635 |
| PP2A Immunoprecipitation Phosphatase Assay Kit | Millipore | Cat# 17-313 |
| APC Annexin V | BD Biosciences | Cat# 550474 |
| Guava TUNEL Kit for Flow Cytometry | Millipore | Cat# 4500-0121 |
| Phospho-Kinase Antibody Array | R&D Systems | Cat# ARY003B |
| NADP/NADPH Assay Kit | Abcam | Cat# Ab65394 |
| Amplex™ Red Glucose/Glucose Oxidase Assay Kit | Thermo Fisher Scientific | Cat# A22189 |
| L-Lactate Assay Kit | Cayman Chemical | Cat# 700510 |
| ATP Bioluminescence Assay Kit CLS II | Roche | Cat# 11699709001 |
| Seahorse XF Glycolysis Stress Test Kit | Agilent Technologies | Cat# 103020-100 |
| Seahorse XF Cell Mito Stress Test Kit | Agilent Technologies | Cat# 103015-100 |
| Deposited Data | ||
| Affymetrix Gene Chip expression data, Ppp2r1a-deletion | This paper | GSE83742 |
| Affymetrix Gene Chip expression data, CEBPA-mediated reprogramming | Dr. Thomas Graf | GSE32330 |
| Chromatin IP sequencing data | ENCODE | GM12878 |
| Expression profiling by high throughput sequencing, Pax5 restoration | Dr. Ross A. Dickins | GSE52870 |
| Affymetrix Gene Chip expression data, mantle cell lymphoma | Dr. Andreas Rosenwald | GSE10793 |
| Affymetrix Gene Chip expression data, mantle cell lymphoma | LLMPP | http://llmpp.nih.gov/MCL |
| Affymetrix Gene Chip expression data, diffuse large B cell lymphoma (DLBCL) | Dr. Louis M. Staudt | GSE10846 |
| Affymetrix Gene Chip expression data, chronic lymphocytic leukemia (B-CLL) | Dr. Tobias Herold | GSE22762 |
| Affymetrix Gene Chip expression data, acute myeloid leukemia | TCGA | N/A |
| Experimental Models: Cell Lines | ||
| KASUMI-2 | DSMZ | Cat# ACC 526 |
| KYO-1 | DSMZ | Cat# ACC 601 |
| EM-2 | DSMZ | Cat# ACC 135 |
| JURL-MK1 | DSMZ | Cat# ACC 532 |
| KCL-22 | DSMZ | Cat# ACC 519 |
| K-562 | ATCC | Cat# CCL-243 |
| MV4-11 | ATCC | Cat# CRL-9591 |
| MOLM-13 | DSMZ | Cat# ACC 554 |
| U-937 | ATCC | Cat# CRL-1593.2 |
| MEG-01 | ATCC | Cat# CRL-2021 |
| BV-173 | DSMZ | Cat# ACC 20 |
| OCI-Ly10 | From Dr. Daniel Hodson | CVCL_8795 |
| SP-53 | From Dr. Ngo Vu | CVCL_C122 |
| H446 | ATCC | Cat# HTB-171 |
| H69 | ATCC | Cat# HTB-119 |
| H526 | ATCC | Cat# CRL-5811 |
| H82 | ATCC | Cat# HTB-175 |
| DMS114 | ATCC | Cat# CRL-2066 |
| DMS273 | Sigma | Cat# 95062830 |
| SBC3 | JCRB | Cat# JCRB0818 |
| BEAS2B | ATCC | Cat# CRL-9609 |
| HEK 293T | Clontech Laboratories | Cat# 632180 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Ppp2r1afl/fl: Ppp2r1atm1.1Wltr | The Jackson Laboratory | Cat# 017441 |
| Mouse: Mb1-Cre: Cd79atm1(cre)Reth | The Jackson Laboratory | Cat# 020505 |
| Mouse: NSG: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | The Jackson Laboratory | Cat# 005557 |
| Mouse: Pax5+/− Ebf1+/− | Dr. Michael Farrar | N/A |
| Oligonucleotides | ||
| Primers for genotyping and qChIP, see Table S6 | Eurofins Scientific | N/A |
| sgRNA for CRISPR-Cas9 mediated gene disruption, see Table S6 | transOMIC Technologies | TEVH-1259980 TEVH-1192838 TEVH-1125696 |
| Recombinant DNA | ||
| MSCV-BCR-ABL1 | Rick Van Etten Laboratory | BCR-ABL1 (p210) |
| MSCV-BCR-ABL1-IRES-Neo | Rick Van Etten Laboratory | BCR-ABL1 (p210) |
| MSCV-NRASG12D-Puro | Müschen Laboratory | NRASG12D |
| MSCV-Luc-Puro | Müschen Laboratory | Firefly luciferase |
| pCL6-Luc-Blast | Müschen Laboratory | Firefly luciferase |
| MSCV-IRES-GFP | Müschen Laboratory | GFP |
| MSCV-Cre-IRES-GFP | Müschen Laboratory | GFP |
| MSCV-PPP2R1A-IRES-GFP | Müschen Laboratory | PP2A subunit A |
| MSCV-CAT-IRES-GFP | Müschen Laboratory | Catalase |
| MSCV-ERT2-IRES-Puro | Müschen Laboratory | Puromycin |
| MSCV-Cre-ERT2-IRES-Puro | Müschen Laboratory | GFP |
| MSCV-ERT2-IRES-GFP | Müschen Laboratory | GFP |
| MSCV-Cre-ERT2-IRES-Puro | Müschen Laboratory | GFP |
| MSCV-mG6pdx-IRES-GFP | Müschen Laboratory | G6PD |
| MSCV-G6PD-IRES-GFP | Müschen Laboratory | G6PD |
| MSCV-FoxO1-ADA-IRES-GFP | Müschen Laboratory | FOXO1 |
| pMIThy1.1(CD90) | From Dr. David A. Fruman | CD90 |
| pMIThy1.1(CD90)-FoxO3a-CA | From Dr. David A. Fruman | CD90 |
| pCGDNsam-IRES-Kusabira Orange | From Dr. H. Phillip Koeffler | K-Orange |
| pCGDNsam-Tigar-IRES-Kusabira Orange | Müschen Laboratory | K-Orange |
| pRetroX-Tet3G-Neo | Clontech Laboratories | Cat# 631188 |
| pRetroX-TRE3G-c/EBPα-Puro | Thomas Graf, Barcelona | CEBPA |
| pRetroX-TRE3G-Pax5-Ikaros-Ebf1-Tcf3 | Müschen Laboratory | Ali’s vector |
| pCL6-ERT2-IRES-GFP | Hassan Jumaa Laboratory | GFP |
| pCL6-PAX5-ERT2-IRES-GFP | Müschen Laboratory | PAX5 |
| pCL6-PAX5-ETV6-ERT2-IRES-GFP | Müschen Laboratory | PAX5-ETV6 |
| pLVX-TRE3G-GFP | Clontech Laboratories | Cat# 631361 |
| pLVX-TRE3G-IKZF1-GFP | Hilde Schjerven laboratory, UCSF | Ikaros |
| pLVX-TRE3G-IK6-GFP | Hilde Schjerven laboratory, UCSF | Dominant-negative Ikaros |
| pTOL-hCMV-TET3G-Hygro | transOMIC Technologies | Cat# CCIHS1001 |
| pCLIP-TRE3G-Cas9-ZsGreen | transOMIC Technologies | Cat# CCIMS1001 |
| pCLIP-hCMV-gRNA-tRFP | transOMIC Technologies | Cat# CCIRS1001 |
| Software and Algorithms | ||
| BRB Array Tool | http://linus.nci.nih.gov/BRB-ArrayTools.html | |
| Gene Cluster | http://www.eisenlab.org/software.html | |
| Java Treeview | http://jtreeview.sourceforge.net/ | |
| Gene Set Enrichment Analysis (GSEA) | http://software.broadinstitute.org/gsea/index.jsp | |
| CompuSyn | http://www.combosyn.com/ | |
| R package “survival” Version 2.35-8 | N/A | |
| Other | ||