Table 1. Mutations identified in SP-2509 drug resistant A673 cells.

Rank	Position	Gene		Loc in gene	Effect	HGVS	Population (%)
1	17q12	MRPL45	mitochondrial ribosomal protein L45	CS	Stopgain	p.Glu53*	88.4%
2	15q13.1	OCA2	OCA2 melanosomal transmembrane protein	splice_site		c.1843-1G>T	45.2%
3	9q34.11	HMCN2	hemicentin 2	CS	NS-SNV	p.Ala352Glu	45.1%
4	1q25.1	SERPINC1	serpin family C member 1	CS	NS-SNV	p.Ser169Ala	44.3%
5	15q11.2	MAGEL2	MAGE family member	CS	NS-SNV	p.Thr1158Pro	42.9%
6	1p34.3	GJB4	gap junction protein beta 4	CS	NS-SNV	p.Asp118Glu	42.2%
7	1p36.12	ALPL	alkaline phosphatase, liver/bone/kidney	CS	NS-SNV	p.Ala503Thr	39.9%
8	1p36.12	E2F2	E2F transcription factor 2	EISR		c.738-9C>A	39.4%
9	5p15.31	SEMA5A	semaphorin 5A	CS	NS-SNV	p.Asp720Glu	39.1%
10	6p12.3	PKHD1	PKHD1, fibrocystin/polyductin	CS	NS-SNV	p.Gly3809Cys	39.0%
11	19q13.12	ZNF781	zinc finger protein 781	CS	Startloss		38.7%
12	16p11.2	APOBR	apolipoprotein B receptor	CS	NS-SNV	p.Leu409Arg	38.7%
13	8p21.3	SH2D4A	SH2 domain containing 4A	CS	NS-SNV	p.Pro89Thr	38.4%
14	17q25.3	CSNK1D	casein kinase 1 delta	CS	NS-SNV	p.Arg279Cys	38.3%
15	1p13.3	LAMTOR5	late endosomal/lysosomal adaptor,	CS	NS-SNV	p.Ala135Asp	36.5%
16	11q23.3	CBL	Cbl proto-oncogene	EISR		c.444-9C>A	36.0%
17	6p21.1	MRPS10	mitochondrial ribosomal protein S10	CS	Stopgain	p.Glu146*	35.6%
18	12q24.13	TPCN1	two pore segment channel 1	CS	NS-SNV	p.Gly779Val	31.4%
19	Xq11.2	MTMR8	myotubularin related protein 8	CS	NS-SNV	Arg541Leu	29.9%
20	6p21.32	HLA-DQA1	major histocompatibility complex, class II, DQ alpha 1	CS	NS-SNV	p.Ser119Tyr	28.3%
21	11p15.5	IRF7	interferon regulatory factor 7	CS	NS-SNV	p.Arg109Leu	27.7%
22	Xq12	HEPH	hephaestin	CS	NS-SNV	p.Tyr789Cys	11.1%
23	14q32.33	AHNAK2	AHNAK nucleoprotein 2	CS	NS-SNV	p.Lys89Asn	11.0%

Abbreviations: CS: Coding sequence, EISR: Extended intronic splice region, NS-SNV: Non-synonymous single nucleotide variant.