Figure 4. OSM increases the migration and proliferation o PDVC57 cells.

(A) In vitro wounds were made in cultures of mitomycin-treated PDVC57 cells and the cells cultured for a further 48 h, with or without OSM, (10 ng/mL). Cell migration into the wounded area was assessed as described in Materials & Methods. Scale bar: 100 μm. Data are presented as the mean ± SEM of the migration area (mm²) from three independent experiments. Mann-Whitney test, ^*p < 0.05. (B) PDVC57 cells were cultured, with or without OSM (10 ng/mL), for 48,h. Cells were fixed and stained with an anti-Ki67 antibody (green) and the cell nuclei stained with DAPI (blue). Scale bar: 100 μm. Data are represented as the mean ± SEM of the percentage of Ki67⁺ cells from three independent experiments. Mann-Whitney test, ^*p < 0.05, ^**p < 0.01.