Figure 6. cSCC development is reduced in OSM-deficient mice.

(A) OSM-KO mice and their WT littermates were subcutaneously injected with 1.10⁶ PDVC57 cells and tumor volume was measured each week using a digital caliper. Data are presented as the mean ± SEM tumor volume (mm³) from five independent experiments, Two-way ANOVA test with Bonferroni post-tests, ^**p < 0.01, ^***p < 0.001. (B) The size of draining lymph nodes were measured under the microscope using a ruler and the volume calculated according to the formula: length x width x thickness. (C) Relative mRNA expression was measured by real-time qPCR in tumors (T) (n = 25) and normal skin (N) (n = 25) of OSM-KO mice and WT littermates. Data are presented as min to max box plots of relative mRNA expression (A.U.). Kruskall-Wallis test with Dunn's Multiple Comparison Test, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.